||22 (Research article)
||Nunn, Brook; Ting, Ying; Malmström, Lars; Tsai, Yihsuan; Squier, Angela; Goodlett, David; Harvey, H
||The path to preservation: Using proteomics to decipher the fate of diatom proteins during microbial degradation
||Limnol. Oceanogr. (2010) 55 1790-1804
||21 citations (journal impact: 3.38)
||We drew upon recent advances in tandem mass spectrometry-based proteomic analyses in order to examine the proteins that remain after a diatom bloom enters the stationary phase precipitates out of the photic zone and is subjected to microbial degradation over a 23-d period within a controlled laboratory environment. Proteins were identified from tandem mass spectra searched against three different protein databases in order to track proteins from Thalassiosira pseudonana and any potential bacterial contributions. A rapid loss of diatom protein was observed over the incubation period 75 of the proteins initially identified were not detected after 72 h of exposure to a microbial population. By the 23rd day peptides identified with high confidence correlated with only four T. pseudonana proteins. Five factors may have influenced the preservation of diatom proteins 1 protection within organelles or structures with multiple membranes 2 the relative cellular abundance in the photosynthetic apparatus 3 the number of transmembrane domains in the protein sequence 4 the presence of glycan modification motifs and 5 the capability of proteins or peptides to aggregate into supramolecules.